Positions

Associate Professor, Biochemistry and Molecular Biology, Brody School of Medicine

Contact Info

zeczyckit@ecu.edu

Publications in VIVO

Co-author Network

Co-investigator Network

Tonya N Zeczycki

member of

selected publications

article
- Oligomeric Changes Regulate Flavin Transfer in Two-Component FMN Reductases Involved in Sulfur Metabolism. Biochemistry. 62:2751-2762. 2023
- Oligomeric Changes are Critical for FMN Reduction and Transfer for Two-component FMN Reductases Involved in Sulfur Metabolism. Biochemistry. 2023
- 2-Aminoimidazole Analogs Target PhoP Altering DNA Binding Activity and Affect Outer Membrane Stability in Gram-Negative Bacteria. Biochemistry. 61:2948-2960. 2022
- Bioenergetic Phenotyping of DEN-Induced Hepatocellular Carcinoma Reveals a Link Between Adenylate Kinase Isoform Expression and Reduced Complex I-Supported Respiration. Frontiers in oncology. 12. 2022
- Morphine Resistance in Spinal Cord Injury-Related Neuropathic Pain in Rats is Associated With Alterations in Dopamine and Dopamine-Related Metabolomics. The journal of pain. 2021
- Racial differences in the limb skeletal muscle transcriptional programs of patients with critical limb ischemia. Vascular medicine (London, England). 1358863-20983918. 2021
- Mitochondrial PE potentiates respiratory enzymes to amplify skeletal muscle aerobic capacity. Science advances. 5. 2019
- (V/K) kinetic isotope effect and steady-state kinetic analysis for the transglutaminase 2 catalyzed deamidation and transamidation reactions. Archives of Biochemistry and Biophysics. 643:57-61. 2018
- 15(V/K) kinetic isotope effect and steady-state kinetic analysis for the transglutaminase 2 catalyzed deamidation and transamidation reactions. Archives of Biochemistry and Biophysics. 643. 2018
- 17-Estradiol Directly Lowers Mitochondrial Membrane Microviscosity and Improves Bioenergetic Function in Skeletal Muscle. Cell Metabolism. 27. 2018
- Docosahexaenoic acid lowers cardiac mitochondrial enzyme activity by replacing linoleic acid in the phospholipidome. The Journal of Biological Chemistry. 293:466-483. 2018
- Extensive skeletal muscle cell mitochondriopathy distinguishes critical limb ischemia patients from claudicants. JCI insight. 3. 2018
- Impact of 17-estradiol on complex I kinetics and HO production in liver and skeletal muscle mitochondria. The Journal of Biological Chemistry. 293:16889-16898. 2018
- Proteolipid domains form in biomimetic and cardiac mitochondrial vesicles and are regulated by cardiolipin concentration but not monolyso-cardiolipin. The Journal of Biological Chemistry. 293:15933-15946. 2018
- The Manganese-Dependent Pyruvate Kinase PykM Is Required for Wild-Type Glucose Utilization by Brucella abortus 2308 and Its Virulence in C57BL/6 Mice. Journal of Bacteriology. 200. 2018
- Kinetic and Thermodynamic Analysis of Acetyl-CoA Activation of Staphylococcus aureus Pyruvate Carboxylase. Biochemistry. 56:3492-3506. 2017
- Multiple non-catalytic ADAMs are novel integrin 4 ligands. Molecular and cellular biochemistry. 2017
- Murine diet-induced obesity remodels cardiac and liver mitochondrial phospholipid acyl chains with differential effects on respiratory enzyme activity. The Journal of nutritional biochemistry. 45:94-103. 2017
- Ceramide-tamoxifen regimen targets bioenergetic elements in acute myelogenous leukemia. Journal of lipid research. 57:1231-42. 2016
- Pyruvate Occupancy in the Carboxyl Transferase Domain of Pyruvate Carboxylase Facilitates Product Release from the Biotin Carboxylase Domain through an Intermolecular Mechanism. Biochemistry. 55:3447-60. 2016
- Increasing levels of cardiolipin differentially influence packing of phospholipids found in the mitochondrial inner membrane. Biochemical and Biophysical Research Communications. 450:366-71. 2014
- Increasing mitochondrial membrane phospholipid content lowers the enzymatic activity of electron transport complexes. Biochemistry. 53:5589-91. 2014
- Nearly 50 years in the making: defining the catalytic mechanism of the multifunctional enzyme, pyruvate carboxylase. The FEBS journal. 281:1333-54. 2014
- Oxamate is an alternative substrate for pyruvate carboxylase from Rhizobium etli. Biochemistry. 52:2888-94. 2013
- Allosteric regulation of the biotin-dependent enzyme pyruvate carboxylase by acetyl-CoA. Biochemical Society transactions. 40:567-72. 2012
- Regulation of the structure and activity of pyruvate carboxylase by acetyl CoA. Archives of Biochemistry and Biophysics. 519:118-30. 2012
- Roles of Arg427 and Arg472 in the binding and allosteric effects of acetyl CoA in pyruvate carboxylase. Biochemistry. 51:8208-17. 2012
- Activation and inhibition of pyruvate carboxylase from Rhizobium etli. Biochemistry. 50:9694-707. 2011
- Interaction between the biotin carboxyl carrier domain and the biotin carboxylase domain in pyruvate carboxylase from Rhizobium etli. Biochemistry. 50:9708-23. 2011
- Novel insights into the biotin carboxylase domain reactions of pyruvate carboxylase from Rhizobium etli. Biochemistry. 50:9724-37. 2011
- Probing the allosteric activation of pyruvate carboxylase using 2',3'-O-(2,4,6-trinitrophenyl) adenosine 5'-triphosphate as a fluorescent mimic of the allosteric activator acetyl CoA. Archives of Biochemistry and Biophysics. 509:117-26. 2011
- Inhibitors of Pyruvate Carboxylase. The open enzyme inhibition journal. 3:8-26. 2010
- Probing the catalytic roles of Arg548 and Gln552 in the carboxyl transferase domain of the Rhizobium etli pyruvate carboxylase by site-directed mutagenesis. Biochemistry. 49:3296-304. 2010
- Insight into the carboxyl transferase domain mechanism of pyruvate carboxylase from Rhizobium etli. Biochemistry. 48:4305-13. 2009
- Highly Cooperative Tetrametallic Ruthenium-mu-Oxo-mu-Hydroxo Catalyst for the Alcohol Oxidation Reaction. Organometallics. 25:1047-1051. 2006

presentations

presentation

principal investigator on

co-principal investigator on

Conference: 37th Annual Gibbs Conference on Biothermodynamics awarded by NSF MCB 2022 - 2023
Calmodulin Kinases and Control of Skeletal Muscle Glucose Metabolism awarded by NIH National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMSD) 2015 - 2020
Biophysical Organization and Kinetics of Cardiolipin Microdomains in Ischemia Reperfusion Injury. awarded by Brody Brothers Endowment Seed Grant 2014
Protecting the Myocardium by Targeting Lipid-Dependent Mitochondrial Respirasomes awarded by Brody School of Medicine Seed Grant Program 2013

investigator on

Organic Cations and Mitochondrial Efficiency awarded by DHHS - National Institutes of Health (NIH) 2020 - 2021
Calmodulin Kinases and Control of Skeletal Muscle Glucose Metabolism awarded by GOV-National Institute of Arthritis & Musculoskeletal & Skin Diseases (NIAMS) 2014

professional service activities

38th Annual Gibbs Conference on Biological Thermodynamics, Chair of Fundraising Committee Chair of Fundraising Committee - oversee all fundraising efforts, submit conference grant (NSF) 2023 - 2024
37th Annual Gibbs Conference on Biological Thermodynamics, Co-Organizer Co-organizer of international conference of biothermodynamics 2022 - 2024
PLoS ONE 2018

education and training

B.S. in Biology and Chemistry, Wisconsin Lutheran College

located in facility