A Novel Regulatory Mechanism for ADAM Metalloproteases Grant uri icon

abstract

  • 1. Optimize the soluble cell binding assays for ADAMs to for quantitative determination of ADAM-integrin binding kinetics. 2. Determine KD and intrinsic binding constants for ADAM28 disintegrin fragment. 3. Using ADAM7 (proposed competitive inhibitor) and ADAM29 (non-competitive control), determine the Ki for ADAM7 disintegrin fragment inhibition of ADAM28 binding. 4. Expand assays to include the full length ADAM ectodomains. 5. Determine Km and kcat/Km for wild-type ADAM28 and a mutant ADAM7 with restored catalytic activity.

date/time interval

  • June 2018 - May 2021