Role of simulated dentinal fluid on dentin collagen remineralization Grant uri icon

abstract

  • The dentinal fluid is an extracellular transudate "lymph" that percolates dentinal tubules, contains serum proteins, immunoglobulins as well as calcium, phosphate, sodium, potassium, and magnesium ions. The concentration of these ions is slightly higher in carious teeth than in sound teeth.2 This indicates that the dentinal fluid is a closed, self-regulating protective system for the tooth organ. If the demineralized collagen fibril scaffold present in caries-affected dentin maintains its structure, then the presence of the dentinal fluid has a key role in providing ions for intra- and extra-fibrillar collagen mineralization and naturally recover its ultra-morphological characteristics. But such role has not been effectively tested neither in vitro nor in vivo. Therefore, our central hypothesizes is that dentinal fluid can remineralize mineral depleted dentin collagen fibrils. Our aim with this proposal is to test in vitro the effect of a simulated dentinal fluid (SDF) on remineralization of acid depleted dentin collagen in artificially created dentin caries lesions under simulated pulpal pressure. Specifically, we will use ultra-high resolution transmission microscopy (HTEM) and elemental analysis to identify and qualify mature crystals formed in acid depleted fibrillar collagen fibrils upon contact with simulated dentinal fluid solution under physiological pulpal pressure for up to a month

date/time interval

  • March 2021 - January 2022